The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device enables researchers to unravel cellular processes in real-time, providing lots of valuable data about the experiments and answers if, when, how, and to what extend the cellular processes occur. CytoSMART’s first fluorescent cell imaging device allows users to track dynamic cellular processes with high specificity by taking high-quality images to create real-time time-lapse movies. Simultaneously, the cells are kept in a controlled environment inside a standard cell culture incubator.
We are delighted to announce that, thanks to our loyal customers, the Lux3 FL has been nominated in the Best New Life Sciences Product of 2020 category!
Great instrument to monitor fluorescently labelled cells.
"In our school we used the Cytosmart Lux3 FL system to monitor GFP expression in green. This works very well, and we obtained nice results and we were also able to see how GFP K.O. cells develop after a Crispr experiment. For RFP it was more difficult as we would like to see gain in expression from GFP to RFP but we have no signal to start with, therefore we were not able to set the settings in a most optimal way to start with. However, we were very pleased to monitor our cells for many days in a row."
Let Lux3 FL capture fluorescent and brightfield images for you
Even when you are not in the lab
Currently, fluorescent labeling is mostly used as an end-point measurement. However, time-lapse imaging of live cells can give much more information about biological processes. By using automated imaging at regular time intervals, the temporal resolution of the fluorescent data is increased, leading to even more relevant data about the cellular processes. In this way, with fluorescence microscopy, researchers can not only determine if a certain process has occurred, but also when it occurred and at what speed.
Live insight in cellular processes
Get the whole story and observe the development of cellular processes while they occur
The CytoSMART Lux3 fluorescence imaging microscope automatically creates time-lapse movies that contain many cellular features. The videos are made from inside the incubator without disturbing your cells and can be immediately accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on your cell cultures and running experiments.
NEW - Quantification of real-time cellular events
Count the number of fluorescent cells over time
For certain experiments, in addition to monitoring cell cultures, it is also required to quantitatively evaluate the progression of cellular events. The CytoSMART Lux3 FL, equipped with a new Object Count algorithm, can automatically calculate the number of fluorescent objects in the image and investigate how this number changes over time. What’s more, the existing software interface has become even more intuitive, allowing users to quickly and easily adjust the parameters of an image.
Working with the CytoSMART microscopes enriches our research on the development of synthetic extracellular matrices, as these microscopes provide convenient, real time, real life information on cell behavior.
Patricia Y.W. Dankers | Professor in Biomedical Materials and Chemistry
Eindhoven University of Technology
Applications of kinetic fluorescence assays
Using fluorescence live-cell imaging has become increasingly valuable in the fields of drug discovery, tissue engineering, immunology, immunotherapy, and cancer research. Various applications, such as monitoring cell viability, determining transfection efficiency, investigating co-cultures, and analyzing cellular processes, can benefit from the fluorescent labels and their detailed kinetic visualization. Many of these applications also benefit from the integrated image analysis included in the CytoSMART Cloud.
Analyze cells in their desired culture environment
Combine fuorescence and brighflield live-cell imaging inside the same incubator
Kinetic fluorescence imaging of cellular processes requires environmental control throughout the experiment. Because of its small size, the Lux3 FL fits inside any standard incubator. This enables you to perform fluorescent live-cell imaging experiments parallel to your regular cell cultures. Because of the remote data accessibility in the CytoSMART Cloud, your cells do not have to be disturbed during the experiment. This makes sure the cells are in the most optimal culture conditions as possible. Next to this, the remote data accessibility prevents you from making unnecessary lab visits.
Simultaneous analysis of multiple cell features
Expand the number of variables you can analyze in your cell culture
Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also provides tools to distinguish cells from each other or distinguish labeled structures within the cells. By tracking changes in the fluorescent signal over time, cell number changes or fluorescently tagged cellular components can easily be identified.
|Channels||Brightfield, green and red fluorescent channels|
|Magnification||10× fixed objective – 20× digital zoom|
|Fluorescence filters||Green – excitation: 452/45 nm; emission: 512/23 nm|
|Red – excitation: 561/14 nm; emission: 630/90 nm|
|Camera||6.4 MP CMOS|
|Data formats||JPG, XLSX, MP4|
|Image size||2072 × 2072 pixels|
|Field of view||1.45 mm × 1.45 mm|
|Culture vessels||Well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels|
|Computer requirements||Windows 10, USB 3.0|
|Dimensions (L × W × H)||166 mm × 140 mm × 135 mm|
|Operating conditions||5 – 40 °C, 20 – 95% humidity|
|Warranty||1-year parts and labor|
|Data storage||Unlimited cloud storage|
|Research use only. Not intended for diagnostic purposes.|
|Extra features||Template functionality (ability to save image acquisition parameters in templates)|
|Object count (count the number of fluorescent objects in the image)|
Lux3 FL brochure 148x148mm spread
Lux3-FL-brochure-148x148mm-sprea ( PDF - 5 MB )
Appnote 0001 Studying the effects of H₂ O₂ on the apoptotic pathway of CHO K1 cells using fluorescence live cell imaging
Appnote-0001-Studying-the-effects-of-H₂O₂-on-the-apoptotic-pathway-of-CHO-K1-cells-using-fluorescence-live-cell-imaging ( PDF - 2 MB )
Appnote 0002 The effect of fibroblast co culture on Hep G2 growth
Appnote-0002-The-effect-of-fibroblast-co-culture-on-HepG2-growth ( PDF - 3 MB )
CASE STUDY Fluorescence imaging 3d cell culture for drug screening
Case-study-Fluorescence-imaging-3d-cell-culture-for-drug-screening_2020-12-03-093515 ( PDF - 1 MB )
Cyto SMART Lux3 FL application guide PDF
CytoSMART-Lux3-FL-application-guide-PDF ( PDF - 8 MB )
CytoSMART Lux3 FL-Apoptosis assay example
lux3-FL-1-pager-example-application ( PDF - 738 KB )
Lux3 FL compatible stainings
Lux3-FL-compatible-stainings ( PDF - 79 KB )
Lux3 FL compatible fluorescent proteins
Lux3-FL-compatible-fluorescent-proteins ( PDF - 117 KB )
Frequently Asked Questions
The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device allows users to track dynamic cellular processes with high specificity by taking high-quality fluorescence images to create real-time, time-lapse movies. Experimental data can be accessed and analyzed remotely via the CytoSMART Cloud portal.
The applications of the Lux3 FL include but not limited to - monitoring cell viability, measuring transfection efficiency, investigating co-cultures, and studying dynamic cellular processes. Researchers working in the fields of drug discovery, tissue engineering, immunology, immunotherapy, and cancer research can benefit from fluorescence live-cell imaging.
Yes, viability is one of the numerous applications of fluorescence live-cell imaging.
There are various fluorescent dyes on the market that can be used to analyze cell viability. Please refer to "Lux3 FL Compatible Staining" PDF in the "Downloads" section to learn more about compatible viability dyes.
Many different fluorescent dyes can be used, as long as the fluorescent dye’s excitation and emission spectra correspond with the fluorescent filters of the Lux3 FL (green – excitation: 452/45 nm, emission: 512/23 nm; red – excitation: 561/14 nm, emission: 630/90 nm). Some examples are propidium iodide (PI) and red fluorescent protein (RFP) for the red channel, and acridine orange (AO), calcein-AM, and green fluorescent protein (GFP) for the green channel.
It is essential to match the fluorescent dye to the optical filter specifications of the device. In addition, it is important to ensure that the dye is not toxic to live cells.
Magnification is equal to a standard microscope with a 10x fixed objective and 20x digital zoom.
Yes, it is possible to set the intensity of the LED for red and green fluorescent channels according to users’ preferences.
Both fluorescent channels are included in the device. Researchers can make time-lapse videos with the brightfield, red and green channel imaging in parallel. Of course, it is also possible to choose just one of the fluorescent channels, depending on the experiment's goal and the researcher's preferences.
Images can be recorded at pre-defined intervals. At the start of a new experiment, you can specify the interval rate anywhere between 5 min and 12 hours.
The CytoSMART Lux3 FL allows monitoring of a wide range of different culture dishes and flasks, such as:
- T-flasks: T-25 up to T-250
- Single well, multi-well plates (6 - 384-well plates)
- Microfluidic chips
- Flat tubes
- Petri dishes
The system requires a dedicated PC or laptop running on Windows 10 or above, with a USB3 port and an active internet connection. If your lab doesn’t have the required hardware, we can supply these for a fee as part of your order.
The CytoSMART Lux3 FL remote functionality runs on cloud-based software. In this cloud-environment, the images and videos are stored and can be accessed using user-specific login details. Next to unlimited data storage, automated image analysis can be performed in the CytoSMART Cloud portal.
The devices are easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the device. Please be aware that the device cannot be autoclaved. After sterilizing with ethanol or IPA, the device can be used in a cleanroom.
This is not necessary. However, to get the best results, we advise covering the fluorescence microscope to minimize the amount of environmental light as much as possible. Turning off the lights in the room can also help to minimize the environmental light.